The present study was undertaken to evaluate the toxicity of fungal contaminated paddy straw in rats causing nervous disorders in goats. Repeated dose 28-day oral toxicity study was conducted in 16 groups consisting of 6 rats of either sex. The fungal culture filtrates of the three isolated fungi C. cladosporoides, P. chartarum and A. niger was administered to induce the toxicity in rats in the dose range of 0.5, 1, 2 ml/ 100 g. Two groups consisting of 6 rats were administered with mixed proportion of all the three isolated fungi C. cladosporoides, P. chartarum and A. niger. During the study period, animals were observed for clinical signs of toxicity. Body weight was measured and blood samples were collected on day 0, 14 and 28 of the study. Biochemical parameters like ALT, AST, BUN, creatinine, and haematological parameters like TLC, TEC, PCV and Hb were analysed. The fungal infected wheat material was analyzed for the presence mycotoxins by LC-MS/MS method.