This volume provides a comprehensive understanding of the methods used in studying phytoplasma diseases and transmission. Chapters cover a wide range of topics, from basic detection methods to advanced genomics and emerging technologies, vectors, plant interactions, and disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Phytoplasma: Methods and Protocols is a valuable resource for all researchers and practitioners with a keen interest in practical methodologies for exploring various facets of phytoplasma disease and its transmission.