Biodiversity is the foundation of life on Earth. It is crucial for the functioning of ecosystems which provide us with products and services without which we couldn't live.Bacteria are the most abundant organisms in the world. Scientists believe that bacteria helped create the earth's atmosphere.It is crucial to know the number and type of Microbes present in this world.For this phylogenetic analysis can be performed by extracting microbial 16S rRNA gene.it has been found that Bacteria helps in Bio-degradation of several harmful chemicals on no wage.Most of the textile industries contain phenol in their wastewater, Analysis show that phenol has serious health hazard when exposure take place to living beings.we can use Bacteria to degrade such phenolic compounds.