To feed the projected population of 1.41 billion by 2025, India needs to produce at least 350 million tonnes of food grains. The expanded food needs of future must be met through intensive agriculture without much expansion in the arable land. The decline in growth rate of productivity of major crops as well as rate of response of crops to added fertilizer under intensive cropping system have possibly resulted from deterioration in physical, chemical and biological quality of soils. Among different organic sources cow urine may be a source of nitrogen. The analysis of cow urine has shown that it contain nitrogen, sulphur, phosphate, sodium, manganese, carbolic acid, iron, silicon, chlorine, salt, vitamins, A, B, C,D & E mineral lactose, enzyme hormone as well as gold acids. Keeping above facts in view the present experiment was conducted to study the effect of nitrogen levels and cow urine on growth and yield of paddy and on available nitrogen status of soil, nitrogen content and uptake of paddy.