The objective of this practical manual is to give a practical knowledge to the researchers, students and to the biotechnologists in different field of life sciences. This manual is a core collection of experiments of biotech sciences, which will be very useful for those who desire to have fundamental practicals. Biotechnology has become a part of our lives. Applications of biotechnological techniques have provided us many benefits, and a better quality of life. The world today uses a language which has a lot of biotechnological science in it. Without knowing, we use many words and phrases derived from biotech science. We are also becoming conscious of our environment as well as our economy. Biotechnology has much to do with both these aspects. It is, therefore, important to learn the language of biotechnological science. Children and adults alike have to know the rudiments of science and must be able to use the language of biotech science where necessary. They must be able to apply the lessons learnt from biotech science in daily life. It is for this purpose that we have produced this practical manual.